Hi Randy,
Hope you are well!
Basically the IBM QUickFile application provide business users with a security -rich, highly reliable way to excharge files-- regardless of size -- inside and outsite the organizations.
What kind of web technologies is the Application under test using?
Have you tried to disable DEP, UAC, Windows firewall during the replay using Ajax TruClient FF or IE protocol?
Let's try to record a new Ajax TruClient IE or FF script, but dsiable the Windows features to see if you can move forward with the execution of the script.
May be there is a limit of the size that the Vugen can handle the file you want to exchange to other point.
Thanks and Regards,